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BAX monoclonal antibody

Product Specifications

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

Swiss Prot

Q07812

Reactivity

Human, Monkey

Immunogen

Purified recombinant human Bax protein fragments expressed in E.coli

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Mitochondrial Control of Apoptosis, Inhibition of Apoptosis, PI3K AKT signaling, SAPK JNK Signaling Cascades, Warburg Effect

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of BAX protein.

Applications Notes

Western blot analysis of BAX expression in COS7 (A), Raji (B), CEM (C), Hela (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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