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ACLY monoclonal antibody

Product Specifications

Background

ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme .

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

ATP-citrate synthase; ATP-citrate (pro-S-) -lyase; ACL; Citrate cleavage enzyme

Swiss Prot

P53396

Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.

Conjugation

Unconjugated

Applications

WB, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of ACLY protein.

Applications Notes

Western blot analysis of ACLY expression in NIH3T3 (A), K562 (B), COS7 (C), Hela (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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