COX4-1 monoclonal antibody

Product Specifications

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane. It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases. The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

Swiss Prot

P13073

Reactivity

Human, Mouse, Rat, Hamster, Goat, Monkey

Immunogen

A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP, FC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of COX4-1 protein.

Applications Notes

Western blot analysis of COX4-1 expression in CHOk1 (A), COS7 (B), NIH3T3 (C), Hela (D), C6 (E), mouse skeletal muscel (F) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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