NRAS Rabbit monoclonal antibody
Product Specifications
Background
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms . Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway . GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
CAS Number
9007-83-4
Structure Composition
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Product Name Alternative
HRAS1; GTPase NRas; Transforming protein N-Ras
Swiss Prot
P01111
Reactivity
Human, Mouse, Rat, Hamster
Immunogen
A synthetic peptide of human Ras
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Tumor Angiogenesis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, NF-kB Signaling, SAPK JNK Signaling Cascades, T cell receptor signaling, TGFβ Smad signaling, Warburg Effect
Applications
WB, IP
Dilution
WB (1/500 - 1/1000), IP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 21 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of NRAS protein.
Applications Notes
Western blot analysis of NRAS expression in Hela (A), CHOK1 (B), C6 (C), NIH3T3 (D), mouse brain (E) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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