EGFR (Phospho-Y1173) Rabbit monoclonal antibody

Product Specifications

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib. Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 . The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

CAS Number

9007-83-4

Structure Composition

Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Product Name Alternative

ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Swiss Prot

P00533

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGFR

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, Tumor Angiogenesis, Jak Stat : IL-6 Receptor Signaling, Phospholipase Signaling

Applications

WB, IHC, IF/ICC, IP

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Phosphorylation

Molecular Weight

~ 160 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of EGFR (pY1173) protein.

Applications Notes

Western blot analysis of EGFR (pY1173) expression in Hela (A), A549 (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Curated Selection

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