E2F1 Rabbit monoclonal antibody
Product Specifications
Background
The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.
CAS Number
9007-83-4
Structure Composition
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Product Name Alternative
RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Swiss Prot
Q01094
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human E2F1
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling, Cell Cycle Control G1 S Checkpoin
Applications
WB, IHC, IF/ICC, IP
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of E2F1 protein.
Applications Notes
Western blot analysis of E2F1 expression in Molt4 (A), C6 (B), NIH3T3 (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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