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Alpha-tubulin 4a Rabbit monoclonal antibody

Product Specifications

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Structure Composition

Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Product Name Alternative

TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain

Swiss Prot

P68366

Reactivity

Human, Mouse, Rat, Hamster

Immunogen

A synthetic peptide of human alpha Tubulin

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Alpha-tubulin 4a protein.

Applications Notes

Western blot analysis of Alpha-tubulin 4a expression in mouse brain (A), NIH3T3 (B), Hela (C), CHOK1 (D), rat brain (E) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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