ALIX Rabbit monoclonal antibody
Product Specifications
Background
Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain. Originally identified as an ALG-2 (apoptosis-linked gene 2) -interacting protein involved in programmed cell death , Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) . Alix has also been shown to be involved in the biogenesis of exosomes, small secreted vesicles that contribute to cell signaling. In addition, Alix interacts with Atg12-Atg3 to promote autophagy and endosomal fusion.
CAS Number
9007-83-4
Structure Composition
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Product Name Alternative
AIP1; ALIX; KIAA1375; Programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95
Swiss Prot
Q8WUM4
Reactivity
Human, Rat, Hamster
Immunogen
Recombinant protein of human ALIX
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 100 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of ALIX protein.
Applications Notes
Western blot analysis of ALIX expression in CHOK1 (A), rat brain (B), Jurkat (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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