AKT (Phospho-S473) Rabbit monoclonal antibody

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis . This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad , forkhead transcription factors, c-Raf , and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway . LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport . In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1 . Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

AMPK Signaling Pathway, Adherens Junction signaling, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease, Tumor Angiogenesis, Regulation of Apoptosis, Tumor Angiogenesis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Regulation of Microtubule Dynamics, Mitochondrial Control of Apoptosis, NF-kB Signaling, PI3K AKT signaling, Protein Kinase C Signaling, T cell receptor signaling, TGFβ Smad signaling, Translational Contral elF4 and p70 S6 Kinase, Warburg Effect, MTOR signaling