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RhoA polyclonal antibody

Product Specifications

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) . RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules. Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility, cytokinesis, focal adhesion assembly and cell polarity.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12

Swiss Prot

P61586

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RhoA. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Adherens Junction signaling, Regulation of Microtubule Dynamics, SAPK JNK Signaling Cascades, TGFβ Smad signaling

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of RhoA protein.

Applications Notes

Western blot analysis of RhoA expression in NIH3T3 (A), mouse lung (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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