NOD2 polyclonal antibody
Product Specifications
Background
The mammalian homologs of the CED-4 proteins, Apaf-1 (CED-4), NOD1 (CARD4) and NOD2, contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker’s A box (P-loop) and B box (Mg2+-binding site) . NOD1 contains a putative regulatory domain and multiple leucine-rich repeats. NOD1 is a member of a growing family of intracellular proteins which share structural homology to the apop_x0002_tosis regulator Apaf-1. NOD1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of NOD1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, NOD1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. NOD2 is composed of two N-terminal CARDs, a nucleotide-binding domain and multiple C-terminal leucine-rich repeats. The expression of NOD2 is highly restricted to mono_x0002_cytes, and activates NFkB in response to bacterial lipopoly-saccharides.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
CARD15; IBD1; Nucleotide-binding oligomerization domain-containing protein 2; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1
Swiss Prot
Q9HC29
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein corresponding to human NOD2. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/200)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of NOD2 protein.
Applications Notes
Western blot analysis of NOD2 expression in mouse kidney (A), rat spleen (B) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Curated Selection
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