IL-10 polyclonal antibody
Product Specifications
Background
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII, leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3. The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1. In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28. IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy. In contrast, increased IL-10 levels contribute to persistent Leishmania major infections.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF
Swiss Prot
P22301
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human IL-10. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of IL-10 protein.
Applications Notes
Western blot analysis of IL-10 expression in Jurkat (A) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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