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KLHL3 polyclonal antibody

Product Specifications

Background

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron . The BCR (KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation . The BCR (KLHL3) complex also mediates ubiquitination and degradation of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney (By similarity) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

KIAA1129; Kelch-like protein 3

Swiss Prot

Q9UH77

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human KLHL3. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 78 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of KLHL3 protein.

Applications Notes

Western blot analysis of KLHL3 expression in U87MG (A), HEK293T (B), Hela (C) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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