ATRIP (Phospho-S68) polyclonal antibody

Product Specifications

Background

In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related) . Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response . Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage . ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex . ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

AGS1; ATR-interacting protein; ATM and Rad3-related-interacting protein

Swiss Prot

Q8WXE1

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRIP with a phosphorylation site at S68. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Phosphorylation

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of ATRIP with a phosphorylation site at S68 protein.

Applications Notes

Western blot analysis of ATRIP (pS68) expression in HCT116 (A), A375 (B), mouse liver (C), mouse muscle (D), rat liver (E), rat muscle (F) whole cell lysates.