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MSK1 (Phospho-S360) polyclonal antibody

Product Specifications

Background

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively . MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region . Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity. These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL

Swiss Prot

O75582

Reactivity

Human, Mouse

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MSK1 with a phosphorylation site at S360. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

NF-kB Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Signaling Pathways Activating p38 MAP Kinase

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Phosphorylation

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of MSK1 with a phosphorylation site at S360 protein.

Applications Notes

Western blot analysis of MSK1 (pS360) expression in mouse liver (A), mouse muscle (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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