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Collagen 4 alpha 4 polyclonal antibody

Product Specifications

Background

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Collagen alpha-4 (IV) chain

Swiss Prot

P53420

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 4 alpha 4. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 164 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Collagen 4 alpha 4 protein.

Applications Notes

Western blot analysis of Collagen 4 alpha 4 expression in A549 (A), PC3 (B), SKOVCAR3 (C), CT26 (D), PC12 (E) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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