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Adenylate Kinase 1 polyclonal antibody

Product Specifications

Background

Adenylate kinases 1-5 (designated AK1-5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling, by catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP) . Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia. Human AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes and is clustered within myofibrils or bound to membranes. AK1-mediated phosphotransfer is essential for maintaining sufficient cellular energy, which enables proper skeletal muscle performance and metabolic activity.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase

Swiss Prot

P00568

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig, Sheep

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adenylate Kinase 1. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Adenylate Kinase 1 protein.

Applications Notes

Western blot analysis of Adenylate Kinase 1 expression in A549 (A), A375 (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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