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PI3K p85 beta polyclonal antibody

Product Specifications

Background

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta

Swiss Prot

O00459

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PI3K p85 beta. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling.

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 95 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of PI3K p85 beta protein.

Applications Notes

Western blot analysis of PI3K p85 beta expression in A549 (A), LO2 (B), BV2 (C), SGC7901 (D), PC12 (E) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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