Anti-verona integron-encoded metallo-β-lactamase (VIM), Rabbit mAb
Product Specifications
Background
Rabbit IgG1, Kappa
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS, pH7.4
Swiss Prot
EME89839.1
Reactivity
VIM-1
Immunogen
MFKLLSKLLVYLTASIMAIASPLAFSVDSSGEYPTVSEIPVGEVRLYQIADGVWSHIATQSFDGAVYPSNGLIVRDGDELLLIDTAWGAKNTAALLAEIEKQIGLPVTRAVSTHFHDDRVGGVDVLRAAGVATYASPSTRRLAEVEGNEIPTHSLEGLSSSGDAVRFGPVELFYPGAAHSTDNLVVYVPSASVLYGGCAIYELSRTSAGNVADADLAEWPTSIERIQQHYPEAQFVIPGHGLPGGLDLLKHTTNVVKAHTNRSVVEHHHHHH
Conjugation
Unconjugated
Applications
WB ELISA
Dilution
WB: 1:500~1:2000 ELISA: 1:5000~10000
Purity
The antibody was affinity-purified by protein A and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Using phage display technology, library of antibody was constructed from peripheral blood of New Zealand rabbits which had been immunized by VIM protein. Screening the library with recombinant VIM protein, the target antibody was obtained and then was purified by recombinant and expression.
Applications Notes
By using Indirect ELISA to detect the binding activity of VIM rabbit monoclonal antibody and recombinant VIM protein. Antigen concentration was 1 µ g/ml.
Host or Source
293F
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