Smad2/3 (phospho-T8) polyclonal antibody

Product Specifications

Background

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Swiss Prot

P84022/Q15796

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, ESC Pluripotency and Differentiation Signaling Pathway, hippo Signaling, hippo Signaling

Applications

WB IF IP

Dilution

WB: 1:1000~1:2000 IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55,60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.

Applications Notes

Western blot (WB) analysis of Smad2/3 (phospho-T8) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα (20ng/ml) for 10 min whole cell lysate Lane3:The Heart tissue lysate of Mouse