Bax Mouse monoclonal antibody
Product Specifications
Background
The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4
Swiss Prot
Q07812
Reactivity
Human, Mouse, Rat
Immunogen
KLH conjugated synthetic peptide derived from human Bax: 31-130/192
Conjugation
Unconjugated
Applications
WB ELISA IHC IF ICC
Dilution
WB: 1:1000-1:1500 ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:200-1:500 ICC: 1:100
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 21 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Bax Mouse monoclonal antibody detects endogenous levels of Bax protein.
Applications Notes
Primary: Anti-Bax at 1/1000 dilution
Host or Source
Mouse
Available Sizes
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