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AQP1 polyclonal antibody

Product Specifications

Background

Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of Aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most Aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP1 is an integral membrane protein expressed in erythrocytes and renal tubule cells.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

Swiss Prot

P29972 (Human) Q02013 (Mouse) P29975 (Rat)

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Aquaporin 1. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB IH IF/IC

Dilution

WB:1:500-1:1,000 IH:1:50-1:100 IF/IC:1:50-1:200

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 25-35 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AQP1 polyclonal antibody detects endogenous levels of AQP1 protein.

Applications Notes

Western blot analysis of Aquaporin 1 expression in mouse kidney (A), mouse liver (B), rat kidney (C), rat liver (D) whole cell lysates.

Host or Source

Rabbit

Available Sizes

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