HSL (phospho-S563) polyclonal antibody
Product Specifications
Background
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
CAS Number
9007-83-4
Structure Composition
PBS with 0.01% thiomersal,50% glycerol, pH7.3.
Product Name Alternative
LIPE; AOMS4; FPLD6; HSL; LHS
Swiss Prot
Q05469
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S563 of rat HSL (XP_006228456.1) .
Conjugation
Unconjugated
Related Pathways
AMPK Signaling Pathway, Insulin &Glucose Signaling
Applications
WB
Dilution
WB: 1:500-1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~81,83KDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HSL (phospho-S563) polyclonal antibody detects endogenous levels of HSL (phospho-S563) protein.
Applications Notes
Western blot analysis of extracts of various cell lines, using HSL (phospho-S563) antibody at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 μM for 20 min) at 37°C.
Host or Source
Rabbit
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