AMPKα1 polyclonal antibody
Product Specifications
Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Swiss Prot
Q13131
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human AMPKα1 (NP_006242.5) .
Conjugation
Unconjugated
Applications
IF/ICC
Dilution
IF/ICC,1:50 - 1:20
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
Refer to figures
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Unmodification
Applications Notes
Immunofluorescence analysis of HeLa cells using AMPKα1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.
Host or Source
Rabbit
Available Sizes
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