Phospho-Histone H2A-S129 polyclonal antibody
Product Specifications
Background
Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
H2A1; SPT11
Swiss Prot
P04911
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around S129 of saccharomyces cerevisiae Histone H2A (NP_010511.3?) .
Conjugation
Unconjugated
Applications
IHC
Dilution
IHC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phosphorylated
Applications Notes
Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H2A-S129 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Host or Source
Rabbit
Available Sizes
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