Phospho-POLR2A CTD-S2 polyclonal antibody

Product Specifications

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

POLR2A; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; Pol II

Swiss Prot

P24928

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A CTD repeatYSPTSPS.

Conjugation

Unconjugated

Applications

WB, IP

Dilution

WB,1:1000 - 1:2000|IP,1:50 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .