Phospho-eIF2α-S51 polyclonal antibody

Product Specifications

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]) .[supplied by OMIM, Feb 2010]

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1

Swiss Prot

P05198

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S51 of human eIF2α (NP_004085.1) .

Conjugation

Unconjugated

Applications

WB, IP, IF/ICC

Dilution

WB,1:1000 - 1:2000|IP,1:50 - 1:100|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .