Phospho-Bcl-2-T56 polyclonal antibody
Product Specifications
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Bcl-2; PPP1R50; BCL2
Swiss Prot
P10415
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T56 of human Bcl-2 (NP_000624.2) .
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB,1:500 - 1:2000|IHC,1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
Refer to figures
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phosphorylated
Applications Notes
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-Bcl-2-T56 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Host or Source
Rabbit
Available Sizes
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