EXO5 polyclonal antibody
Product Specifications
Background
The protein encoded by this gene is a single-stranded DNA (ssDNA) -specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage. [provided by RefSeq, Nov 2016]
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
EXO5; C1orf176; DEM1; Exo V; hExo5; ExoV
Swiss Prot
Q9H790
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein of human EXO5 (NP_073611.1) .
Conjugation
Unconjugated
Applications
WB
Dilution
WB,1:500 - 1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
42kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Unmodification
Applications Notes
Western blot analysis of extracts of various cell lines, using EXO5 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 1s.
Host or Source
Rabbit
Available Sizes
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