EIF4A polyclonal antibody

Product Specifications

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss Prot

P60842/Q14240/P38919

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human eIF4A (NP_001407.1) .

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .