BRK1 polyclonal antibody

Product Specifications

Background

Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

BRK1; C3orf10; HSPC300; MDS027; hHBrk1

Swiss Prot

Q8WUW1

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human BRK1 (NP_060932.2) .

Conjugation

Unconjugated

Applications

IHC, IF/ICC

Dilution

IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

Refer to figures

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Unmodification

Applications Notes

Immunohistochemistry of paraffin-embedded human breast cancer using BRK1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.