ALOX12 polyclonal antibody

Product Specifications

Background

Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. Mainly converts arachidonate ((5Z,8Z,11Z,14Z-eicosatetraenoate to the specific bioactive lipid (12S-hydroperoxyeicosatetraenoate/ (12S-HPETE. Through the production of bioactive lipids like (12S-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S-hydroperoxy-docosahexaenoate/ (14S-HPDHA resulting in the formation of (13S,14S-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoate to generate specialized pro-resolving mediators (SPMs like resolvin D5 ((7S,17S-diHPDHA and (7S,14S-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ALOX12;12-LOX;12S-LOX; LOG12;12S-type

Swiss Prot

P18054

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human ALOX12 (NP_000688.2) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .