Phospho-CFL1-Y139 polyclonal antibody
Product Specifications
Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CFL1; CFL; HEL-S-15; cofilin; cofilin-1
Swiss Prot
P23528
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1) .
Conjugation
Unconjugated
Applications
IHC
Dilution
IHC,1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phosphorylated
Applications Notes
Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Host or Source
Rabbit
Available Sizes
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