Phospho-c-Abl-Y412 polyclonal antibody

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t (9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410) . Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-c-Abl-Y412 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.