Phospho-Src (Tyr530) monoclonal antibody
Product Specifications
Background
Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2) .
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Swiss Prot
P12931
Reactivity
Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src
Conjugation
Unconjugated
Applications
WB, IP
Dilution
WB: 1/500-1/1000 IP: 1/20
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 60 kDa; Observed MW: 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-Src in rat lung lysates using Phospho-Src antibody.
Host or Source
Rabbit
Available Sizes
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