Phospho-Rb (Thr356) monoclonal antibody
Product Specifications
Background
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Swiss Prot
P06400
Reactivity
Human, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr356 of human Rb
Conjugation
Unconjugated
Applications
WB, IHC-P, IP
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 106 kDa; Observed MW: 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Rb in HL-60, U2OS, C6 lysates using Phospho-Rb antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
![PD-L1 (CD274) Human Monoclonal Antibody [Clone ID: OTI8C8]](https://cdn.genprice.uk/products/937/68dd18bb061270361e908e70/imageurl/cf813015.webp)
CF813015 100 µg
PD-L1 (CD274) Human Monoclonal Antibody [Clone ID: OTI8C8]
Sign In for Pricing
View Details 
![Ly6g Rat Monoclonal Antibody [Clone ID: NIMP-R14]](https://cdn.genprice.uk/products/937/68dd189c061270361e8fcb8b/imageurl/am26331pu-n.webp)
AM26331PU-N 100 µg
Ly6g Rat Monoclonal Antibody [Clone ID: NIMP-R14]
Sign In for Pricing
View Details 