Phospho-Rb (Thr252) monoclonal antibody
Product Specifications
Background
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Swiss Prot
P06400
Reactivity
Human, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr252 of human Rb
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 106 kDa; Observed MW: 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Rb in HL-60, U2OS, C6 lysates using Phospho-Rb antibody.
Host or Source
Rabbit
Available Sizes
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