Phospho-Jun/JunD (Ser73/Ser100) monoclonal antibody
Product Specifications
Background
C-jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUND; Transcription factor jun-D
Swiss Prot
P05412/P17535
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser73 of human Jun
Conjugation
Unconjugated
Applications
WB, IP
Dilution
WB: 1/500-1/1000 IP: 1/20
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 36 kDa; Observed MW: 38,42,48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Jun /JunD in A549, HL-60, U251, U87-MG lysates using Phospho-Jun/JunD antibody.
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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