Phospho-HSF1 (Ser303/Ser307) monoclonal antibody
Product Specifications
Background
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1
Swiss Prot
Q00613
Reactivity
Human
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser303/Ser307 of human HSF1
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 57 kDa; Observed MW: 82 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of HSF1 in A549, HL-60, U2OS lysates using Phospho-HSF1 antibody.
Host or Source
Rabbit
Available Sizes
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