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Phospho-Histone H3 (Ser10/Thr11) monoclonal antibody

Product Specifications

Background

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

H3 histone; family 3A; H3 histone; family 3B (H3.3B) ; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3

Swiss Prot

P68431

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 15 kDa; Observed MW: 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Immunocytochemistry analysis of Phospho-Histone H3 in Hela using Phospho-Histone H3 antibody, and DAPI.

Host or Source

Rabbit

Available Sizes

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