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Acetyl-Histone H3 (Lys27) monoclonal antibody

Product Specifications

Background

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

H3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

Swiss Prot

P68431

Reactivity

Human

Immunogen

A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, IP, ChIP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20

Purity

Affinity Purified

Modification

Acetylated

Molecular Weight

Calculated MW: 15 kDa; Observed MW: 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Histone H3 in Hela, A549, HL-60 lysates using Acetyl-Histone H3 antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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