Phospho-Raf1 (Ser259) monoclonal antibody

Product Specifications

Background

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Swiss Prot

P04049

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1

Conjugation

Unconjugated

Applications

WB, IHC-P

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 73 kDa; Observed MW: 73 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-Raf1 in K562, C6, 3T3, Hela lysates using Phospho-Raf1 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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