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Phospho-PKA RII alpha (Ser99) monoclonal antibody

Product Specifications

Background

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit

Swiss Prot

P13861

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser99 of human PKA R2

Conjugation

Unconjugated

Applications

WB, IHC-P, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 46 kDa; Observed MW: 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-PKA RII alpha in 3T3, Raw264.7 lysates using Phospho-PKA RII alpha antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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