Phospho-p27 Kip 1 (Ser10) monoclonal antibody
Product Specifications
Background
The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Swiss Prot
P46527
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Conjugation
Unconjugated
Applications
WB, IHC-P, IP
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 22 kDa; Observed MW: 27 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-p27 KIP 1 in Hela, 3T3 lysates using Phospho-p27 Kip 1 antibody.
Host or Source
Rabbit
Available Sizes
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