Phospho-HSF1 (Ser326) monoclonal antibody

Product Specifications

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

Swiss Prot

Q00613

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser326 of human HSF1

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 57 kDa; Observed MW: 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-HSF1 in K562, Hela lysates using Phospho-HSF1 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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