Phospho-Histone H3 (Ser28) monoclonal antibody

Product Specifications

Background

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

H3 histone; family 3A; H3 histone; family 3B (H3.3B) ; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3

Swiss Prot

P68431

Reactivity

Human, Mouse

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 15 kDa; Observed MW: 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Histone H3 in 3T3, Hela lysates using Phospho-Histone H3 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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