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Phospho-eIF4E (Ser209) monoclonal antibody

Product Specifications

Background

EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Swiss Prot

P06730

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, IP

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 25 kDa; Observed MW: 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of eIF4E in K562, rat Brain, C6, 3T3 lysates using Phospho-eIF4E antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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