Phospho-MDM2 (Ser166) monoclonal antibody
Product Specifications
Background
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
Double minute 2 protein; Hdm2; Oncoprotein Mdm2
Swiss Prot
Q00987
Reactivity
Human, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 55 kDa; Observed MW: 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-MDM2 in Jurkat, C6, Hela lysates using Phospho-MDM2 antibody.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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