Phospho-PKC alpha (Thr638) monoclonal antibody
Product Specifications
Background
PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.
CAS Number
9007-83-4
Structure Composition
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Product Name Alternative
PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
Swiss Prot
P17252
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha
Conjugation
Unconjugated
Applications
WB, IHC-P, IP
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 77 kDa; Observed MW: 80 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-PKC alpha in C6, C6+TPA, 3T3, 3T3+TSA lysates using Phospho-PKC alpha antibody.
Host or Source
Rabbit
Available Sizes
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