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Phospho-Smad3 (Ser423/425) monoclonal antibody

Product Specifications

Background

Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad) . Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Swiss Prot

P84022

Reactivity

Human, Rat, Hamster

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser423/Ser425 of human Smad3

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1/500-1/1000

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 48 kDa; Observed MW: 52 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-Smad3 in Jurkat, C6, CHO-K1, Hela lysates using Phospho-Smad3 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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